We separate molecules and unite people.
Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography systems and components.
With 130 employees, we are an established manufacturer of HPLC systems and components. Our valves, pumps, detectors, and other components are used for many separation processes outside of HPLC and are popular with customers who want custom solutions (OEM). KNAUER is an ideal partner for those who want more than standard solutions can provide.
Furthermore, the KNAUER Academy offers courses on HPLC and application development.
We support technological advancement today and in the future.
KNAUER develops and manufactures scientific instruments of superior quality for liquid chromatography systems and components, including:
AZURA components are combinable and highly adaptable. They enable users to cover one of the widest LC ranges. Compact HPLC for standard tasks, extended range HPLC Plus (max. 700 bar) and UHPLC (max. 1000 bar) with optimized flow paths provide for most sensitive and high resolution analysis. A choice of flow cells and pump heads that differ in performance and material provides excellent application flexibility.
From simple to complex, from Lab to Pilot scale: Design your AZURA® FPLC system according to your purification task!
AZURA Bio purification platform allows you to create FPLC systems with highest independence. Just pick your modules and build-up the system yourself. Continue flexibility with intuitive PurityChrom® software.
SMB is a method in process chromatography that enables substance mixtures to be continuously separated and extracted in two fractions. Its efficiency is significantly higher than batch chromatography, through better utilization of the column stationary phase.
Due to their modular design, AZURA SMB systems can also be configured for other multi-column processes. KNAUER offers SMBC systems for purification up to 1000 kg/year.
KNAUER launched a single quadrupole mass spectrometer. With the KNAUER 4000 MiD® the Berlin-based company expands their preparative HPLC portfolio to the field of mass directed purification. The usage of mass spectrometry in the preparative HPLC enables the collection of target molecules according to their molecular mass. Natural products, pharmaceuticals, and other small organic molecules can be purified in a time- and cost-saving manner.
The KNAUER 4000 MiD covers a mass range of 50 to 800 m/z. Due to the integrated vacuum system, the 4000 MiD has a very small footprint and can be operated in laboratories with limited space. Together with the MiDas™ automated sampling unit, the 4000 MiD enables high-throughput preparative HPLC. The AZURA® Prep HPLC for mass directed fractionation is controled with ClarityChrom®, an easy-to-use chromatography data system for workstations.
The family-owned company KNAUER has more than 55 years experience in liquid chromatography and provides a large portfolio of solutions for preparative HPLC applications. KNAUER is an ISO 9001 certified company with strict manufacturing and process reliability for highest quality "Made in Germany".
Please refer to www.knauer.net/mid for more information on the KNAUER 4000 MiD.
KNAUER Wissenschaftliche Geräte GmbH
Hegauer Weg 38
The Berlin-based manufacturer of laboratory measuring instruments KNAUER Wissenschaftliche Geräte GmbH reported today, that its high-pressure metering pumps played an important role in the development of the groundbreaking new manufacturing process of methanesulfonic acid (MSA) directly from methane and sulfur trioxide, which was successfully realized by researchers from Duisburg's Grillo-Werke AG .
Currently methanesulfonic acid is already produced on an industrial scale. Its strongly acidic but non-oxidizing properties make it an interesting catalyst for many reactions in organic chemistry, especially in polymerizations. Methanesulfonic acid is also used in electroplating and increasingly also in industrial cleaners, where it is used as a substitute for phosphoric, hydrochloric or sulfuric acid, because it is less harmful to the environment at comparable or greater efficacy than the mineral acids used previously. The preparation of methanesulfonic acid is currently relatively expensive, because of multi-stage processes, which are partly dependent on toxic intermediates such as dimethyl disulfide.
The direct reaction of methane with sulfur trioxide would be the most obvious solution. Researchers worldwide have long been experimenting with the direct activation of methane. As early as in the 1950s, a US patent was filed using sulfur trioxide, however, the achieved yields of methanesulfonic acid were low and the number of unwanted by-products high. 
The Grillo researchers took a different approach than most other researchers. They relied on relatively mild temperatures below 100 degrees Celsius and pressures around 50 bar only. The cunning in their process is an activator molecule that triggers sulfonation and can easily be re-synthesized from MSA and sulfuric acid in a secondary process. By the way, sulfuric acid serves as a solvent in the process.
A tricky part of the new MSA synthesis is the precise metering of sulfur trioxide (SO3). Sulfur trioxide is only liquid in a narrow temperature range between 32°C and 44°C, reacts violently when in contact with water and chars most plastics very quickly. This means that it is very difficult to handle.
"In search of suitable pumps for this task, we had already received a few cancellations from suppliers before turning to KNAUER. KNAUER also could not offer us a model off the shelf, but they were the only manufacturer to embark on a little development trip," said Dr. Timo Ott, Head of Product and Process Development for the chemistry branch at Grillo.
KNAUER high-pressure dosing pumps are based on HPLC technology and inherently provide high chemical resistance, excellent flow rate precision, and low pulsation. However, pumping liquid sulfur trioxide required customization, especially with regard to plastic parts in the flow path. Another important prerequisite for the success was the powerful pump head heating offered by KNAUER in order to keep sulfur trioxide fluid even at low flow rates or necessary stops.
"It is part of our company philosophy that we find beneficial solutions for our customers and together with them. We are particularly pleased that we were able to make our contribution to the success of Grillo’s MSA synthesis, which is in my opinion a research project of global importance,” states Dr. Christian Benkhäuser, Head of Hardware Development at KNAUER.
The special feature of the methanesulfonic acid production process developed by the Grillo researchers is that it works much more efficiently than previous processes and makes methane usable as a carbon source for sulfonation. Certainly, with this process methane is becoming more important as a raw material. Wasting methane through flaring will hopefully become less common. Using biogas as a source of methane, the process can do without a fossil carbon source. A recently published comparative life cycle assessment (LCA) study from the University of Zurich concludes that the new process has a three times smaller environmental impact than previous methods. Greenhouse gas emissions are 85% lower. 
"When the new manufacturing process for methanesulfonic acid will be introduced on an industrial scale, the environment will benefit of it," Dr. Ingo Biertümpel is convinced. He is Head of R & D in the Chemistry Branch of Grillo-Werke AG and the company’s Sustainability Officer.
Something else is already clear today: It is worthwhile, as Grillo has done here, to leave the beaten path, it is also worthwhile, like KNAUER, not to say hastily "we can’t deliver that" and it is fun working together to develop new solutions.
More about KNAUER dosing pumps at: www.knauer.net/dosing
The family-owned business KNAUER Wissenschaftliche Geräte GmbH from Berlin with around 135 employees looks back on more than 55 years of company history and is one of the pioneers of high-performance liquid chromatography (HPLC) in Germany. In parallel with the chromatography activities, HPLC pumps have repeatedly been used as high-pressure metering pumps. For more than ten years now, KNAUER treats dosing pumps as a business segment. The ISO 9001 and ISO 14001 certified company with its strict manufacturing and process reliability stands for highest quality "Made in Germany" and for acting environmentally conscious as a business. www.knauer.net
Grillo-Werke AG is a family-owned company with headquarters in Duisburg, which was founded in 1842 and is one of the market leaders in the processing of zinc and sulfur in Europe. Today, the globally operating group is divided into the divisions Chemicals, Zinc Oxide, Metals and RHEINZINK with approx. 1,600 employees. The product portfolio of the chemical division includes sulfur chemicals, acids, sodium and zinc salts as well as zinc oxides for a wide range of applications. According to the guiding principle of "progress by tradition", Grillo attaches great importance to the continuous improvement of its products and processes with respect for the environment and society. www.grillo.de
Press release Grillo-Werke AG: 2016-06-20, https://grillo.de/?page_id=9446546677
John C. Snyder, Aristid V. Grosse, Houdry Process Corp, https://patents.google.com/patent/US2493038A/en
Steve Kappenthuler, Sandro Olveira, Jonathan Wehrli, Stefan Seeger, Environmental assessment of alternative methanesulfonic acid production using direct activation of methane Journal of Cleaner Production, Volume 202, 20 November 2018, Pages 1179-1191, https://doi.org/10.1016/j.jclepro.2018.07.284
KNAUER Wissenschaftliche Geräte GmbH, Hegauer Weg 38, 14163 Berlin
+49 30 809 727-0, firstname.lastname@example.org, www.knauer.net
Press contact: Oliver Gültzow, email@example.com, +49 30 809727-43
The new Sepapure® columns and media represent high-quality alternatives for common protein purification tasks like size-exclusion (SEC), affinity (AC) and ion-exchange (IEX) chromatography.
Sepapure FPLC columns come as prepacked ready-to-use 1 ml or 5 ml cartridges and can also be purchased as bulk material. The media for affinity as well as ion-exchange chromatography are based on agarose, which is well-known in FPLC chromatography.
For affinity chromatography the agarose solid phase is functionalized with ligands for the purification of recombinantly expressed proteins with either His- or GST-tags. Antibodies can be purified via Protein A or Protein G affinity. The ion-exchange columns can be purchased as weak and strong anion or cation exchangers. The basis of our Sepapure desalting column is dextran with a 5 kDa exclusion limit, which reliably separates small molecules and salts from the larger valuable proteins.
KNAUER Sepapure columns are made to fit perfectly with AZURA FPLC systems but can also be used with most other FPLC systems on the market.
Learn more about the new FPLC columns at www.knauer.net/sepapure
KNAUER has over 56 years of experience in developing and manufacturing scientific instruments. Providing labs with equipment that fits the user’s needs has always been the main goal of the family-owned business. The company’s LC systems for protein purification are known for high precision, robustness, and scalability due to a modular design. KNAUER is an ISO 9001 certified company with strict manufacturing and process reliability for highest quality "Made in Germany". Environmental protection is an important concern of the company. Environmental management in accordance with ISO 14001, green electricity and its own solar power plant are just a few examples.
KNAUER Wissenschaftliche Geräte GmbH, Hegauer Weg 38, 14163 Berlin, Germany
+49 30 809727-0, +49 30 8015010 (Fax), firstname.lastname@example.org, www.knauer.net
Press contact: Oliver Gültzow, email@example.com, +49 30 809727-43